oligo dt 25 beads (New England Biolabs)

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New England Biolabs oligo dt 25 beads
Oligo Dt 25 Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligo+dt+25+beads/Magnetic+mRNA+Isolation+Kit/pmc12997395-110-5-11
Average 97 stars, based on 617 article reviews

oligo dt 25 beads - by Bioz Stars, 2026-10

97/100 stars

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Purification:

Article Title: Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection.
Article Snippet: .. Poly(A) RNA was purified using 100 μL of oligo(dT)25 beads (New England Biolabs, #S1419S) following the procedure described in the ‘ivRIC’ section, but eluting in 100 μL. .. Eluted RNA concentration was measured in a Qubit 2.0 Fluorometer using Qubit RNA High Sensitivity Assay Kit (Thermo Fisher Scientific, #Q32855), and HIV-1 RNA at different sample dilution was quantified using the Luna Universal One-step RT-qPCR kit (New England Biolabs, #E3005S) with specific primers against the 5′ end of HIV-1 gRNA (Key Resources Table).

Article Title: Febrile temperature activates the innate immune response by promoting aberrant influenza A virus RNA synthesis.
Article Snippet: .. After extraction, 1 μg of RNA was either treated or mock- treated with oligo(dT)25 beads from the magnetic mRNA isolation kit (NEB) according to the manufacturer’s instructions and purified using the RNA Clean & Concentrator- 5 kit (Zymo Research). ..

Article Title: Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection
Article Snippet: .. Poly(A) RNA was purified using 100 μL of oligo(dT) 25 beads (New England Biolabs, #S1419S) following the procedure described in the ‘ivRIC’ section, but eluting in 100 μL. .. Eluted RNA concentration was measured in a Qubit 2.0 Fluorometer using Qubit RNA High Sensitivity Assay Kit (Thermo Fisher Scientific, # Q32855 ), and HIV-1 RNA at different sample dilution was quantified using the Luna Universal One-step RT-qPCR kit (New England Biolabs, #E3005S) with specific primers against the 5′ end of HIV-1 gRNA (Key Resources Table).

Article Title: Febrile temperature activates the innate immune response by promoting aberrant influenza A virus RNA synthesis
Article Snippet: .. After extraction, 1 μg of RNA was either treated or mock-treated with oligo(dT) 25 beads from the magnetic mRNA isolation kit (NEB) according to the manufacturer’s instructions and purified using the RNA Clean & Concentrator-5 kit (Zymo Research). ..

Incubation:

Article Title: RNA methyltransferase NSUN2 enhances vasculogenic mimicry and malignant progression of cervical cancer through upregulation of MMP-9
Article Snippet: .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.). .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.).

Article Title: RNA methyltransferase NSUN2 enhances vasculogenic mimicry and malignant progression of cervical cancer through upregulation of MMP‑9
Article Snippet: .. Total RNA was incubated with oligo dT(25) beads (cat. no. S1550S; New England Biolabs, Inc.) for 10 min at RT and then washed with Lysis/Binding buffer (cat. no. B1556AVIAL; New England Biolabs, Inc.), Wash Buffer I (cat. no. B1558AVIAL; New England Biolabs, Inc.), Wash Buffer II (cat. no. B1559AVIAL; New England Biolabs, Inc.)and Low Salt Buffer (cat. no. B1557AVIAL; New England Biolabs, Inc.). .. The mRNA was eluted with Elution Buffer (cat. no. B1561AVIAL; New England Biolabs, Inc.) and samples containing 300 or 600 ng of mRNA were transferred to nylon membranes.

Lysis:

RNA Sequencing:

Article Title: Combined metabolome and transcriptome reveal HmF6’H1 regulating simple coumarin accumulation against powdery mildew infection in Heracleum moellendorffii Hance
Article Snippet: Subsequently, a quantity of approximately 50 mg of frozen tissue was utilized for the extraction of total RNA using an OmniPlant RNA Kit (CWBIO, China), following the instructions provided. .. In the RNA-seq analysis, the NEBNext, Oligo (dT)25 beads from NEB, USA were employed to selectively enhance the presence of mRNA in a 50ul total RNA sample. .. Subsequently, a NEBNext, UltraRNA Library Prep Kit (Biolabs, China) for Illumina from NEB was utilized to create an mRNA library from these enriched samples, following the provided instructions.

Article Title: Combined metabolome and transcriptome reveal HmF6'H1 regulating simple coumarin accumulation against powdery mildew infection in Heracleum moellendorffii Hance.
Article Snippet: Subsequently, a quantity of approximately 50 mg of frozen tissue was utilized for the extraction of total RNA using an OmniPlant RNA Kit (CWBIO, China), following the instructions provided. .. In the RNA-seq analysis, the NEBNext, Oligo (dT)25 beads from NEB, USA were employed to selectively enhance the presence of mRNA in a 50ul total RNA sample. .. Subsequently, a NEBNext, UltraRNA Library Prep Kit (Biolabs, China) for Illumina from NEB was utilized to create an mRNA library from these enriched samples, following the provided instructions.

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